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K13 A212T mutants did not alter OZ439 susceptibility but retained resistance to DHA when combined with R539T or C580Y in RSAs To characterize the contribution of K13 A212T - alone and in combination with the pre-existing R539T mutation or with K13 C580Y - to parasite susceptibility to OZ439, we used CRISPR/Cas9 genome editing to insert or remove the A212T mutation from parental Cam3.II parasites and resistant clones generated by in vitro selection (fig

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