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A third mechanism was the chemical modification of the PBD’s structure by a reduction in one of the imine functional groups, thus changing DNA crosslinking PBD-dimer to a DNA-alkylating metabolite named Indolinobenzodiazepine dimers (IGN) that resulted in a more efficient release of the payload with better efficacy in murine tumor models, where mice tolerated IGN-conjugated ADCs at a higher dose [42,43]