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Injection Site Necrosis and Ulceration Following Vaccination in an Adult Patient - JDDonline - Journal of Drugs in Dermatology
the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95° C
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The mouse hemorrhagic liver model was established as follows: Balb/c mice were anesthetized and then fixed on the mouse operating bed
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