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To this end, the DNA oligo pool was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491) in 48 parallel 50 µl PCR reactions, each containing 10 μL 5X Q5 Reaction Buffer, 1 μL dNTP (10 mM each), 2.5 μL forward primer (10 μM), 2.5 μL reverse primer (10 μM), 1 ng oligo pool template, and 0.5 μL Q5 High-Fidelity Polymerase, using the following thermocycler conditions: 98 °C for 30 s
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doi: 10.1080/15548627.2021.1920814
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