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Diverticulitis | New England Journal of Medicine

(12)制备Fmoc-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂： (12) Preparation of Fmoc-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: 在步骤(11)的Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂中，加入脱帽试剂10～50℃反应5～30分钟，用真空泵抽干，重新加入脱帽试剂10～50℃反应10～60分钟，抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，加入用DMF溶解的Fmoc-Pro-OH、DIEA、TBTU/HOBt或HBTU/HOBt或BOP/HOBt的混合物，10～50℃反应10～60分钟，用真空泵抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，获得Fmoc-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂； In the Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin in step (11), add the decapping reagent and react at 10～50°C 5 to 30 minutes, dry with a vacuum pump, re-add the decapping reagent at 10 to 50 ° C for 10 to 60 minutes, dry, wash with isopropanol for 3 times, DMF for 3 times, re-distilled DMF for 2 times, add Dissolve Fmoc-Pro-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt mixture in DMF, react at 10-50°C for 10-60 minutes, dry with vacuum pump, wash with isopropanol 3 times, DMF Washed 3 times, washed 2 times with re-distilled DMF, and dried to obtain Fmoc-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu- Val-resin

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Grace Whitmer is an inpatient senior clinical dietitian at UT MD Anderson