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OS causes aberrant signaling pathways which can hyperphosphorylate the microtubule protein τ, leading to the abnormal formation of NFTs in the AD brain
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15 µg of lysates were reduced in NuPAGE reducing reagent (Invitrogen) and boiled for 5 min prior to loading in 4-12% NuPAGE Bis-Tris Gel for separation by electrophoresis
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