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coli HT115 containing L4440 empty vector or the indicated cDNA fragment in L4440 was inoculated in 100 ml of LB containing 100 μg/ml ampicillin and incubated overnight at 37 °C in a shaking incubator at 250 rpm

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But for molecules at the surface there are few adjacent molecules above them, only below, so all of the attractive forces are directed inwards, away from the surface (Figure 5.1.6)