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The cell lysate was then incubated with Ni-NTA beads, and the beads were then washed with 20 mM Tris pH 7.8, 500 mM NaCl, 20 mM imidazole and 5% glycerol, and the protein was subsequently eluted with 20 mM Tris pH 7.8, 500 mM NaCl, 250 mM imidazole and 5% glycerol

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