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SYBR Green (Bio-Rad Laboratories, Veenendaal, Netherlands) was employed as a double-stranded DNA (dsDNA) binding dye according to a previously described protocol (20, 27, 81) with the bacterium-specific primers Bart_1Fq 5- GAATGTTAGCCGTTGGTAGG -3 and Bart_1R 5- GCAGCACCTGTCTCCGAC -3, amplifying 200 bp of the 16S rRNA