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Load the soluble fusion protein to the equilibrated glutathione Sepharose column using a flow rate of 0.1 ml/min (see Notes 21 and 22)
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[DOI] [PMC free article] [PubMed] [Google Scholar] 3.Bjørklund G., Shanaida M., Lysiuk R., Butnariu M., Peana M., Sarac I., Strus O., Smetanina K., Chirumbolo S
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