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The glutathione assay method utilizes an adaptation of Ellman’s Reagent (DTNB), where 5,5’-dithiobis (2-nitrobenzoic acid) (DTNB) reacts with GSH to form the compound TNB (2-nitro-5-thiobenzoic acid), which is then quantified using HPLC analysis
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In addition, chitosan coating improves the gastric stability of curcumin-loaded liposomes and helps retain curcumin under simulated gastric conditions compared with uncoated formulations 14 , which may partly contribute to the observed 23.2-fold increase in dose-normalized bioavailability
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