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Some of the genes having an altered expression belonged to cellular processes such as apoptosis, cell cycle, inflammation, and metabolic process, stress genes, signal transduction, and metabolic process

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When we seeded Cas9-expressing HCT-8 cells (induced using 0.5 μg/mL doxycycline) along with transfection reagent Dharmafect 2 and 10 nM tracrRNA into wells containing 10 nM pooled guides against a particular gene, we found protein levels reduced by 70-80 % three days later (Fig S1D)

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