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Fill the line with distilled water Make a 10-fold dilution of the 1 mg/ml BSA stock for a working 100 µg/ml BSA standard Using the 1.5mL microtubes, prepare the calibration solutions as outlined in the table below Method To each 100 µl sample, add 1 ml of Bradford reagent Wait at least 5 mins for the color to develop Make sure the Open Colorimeter has the 595 nm LED installed Turn on the Open Colorimeter and select absorbance Blank the colorimeter with distilled water Measure absorbance of all of the solutions Part 2: Importing protein calibration data to Open Colorimeter In this section we will import the Bradford protein assay calibration data into the Open Colorimeter so it can be easily accessed to measure protein concentrations in samples