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Forward scatter (FSC) and side scatter (SSC) are used to gate out (remove from analysis) unwanted events such as dead cells or doublets (when two cells pass together but the cytometer counts it as a single event)
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27,49 Consequently, in contrast to sites such as the skin and gut, which have robust and self-sustaining microbiomes, the lung microbiome may migrate from adjacent sites such as the oropharynx and the URT
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